By Lijie Grace Zhang, John P Fisher, Kam Leong
3D Bioprinting and Nanotechnology in Tissue Engineering offers a close advent to those applied sciences and their commercial purposes. Stem cells in tissue regeneration are coated, in addition to nanobiomaterials. Commercialization, criminal and regulatory issues also are mentioned with a view to assist you translate nanotechnology and 3D printing-based items to and the sanatorium. Dr. Zhang’s and Dr. Fishers’ staff of specialist participants have pooled their services with the intention to supply a precis of the suitability, sustainability and barriers of every procedure for every particular program. The expanding availability and reducing bills of nanotechnologies and 3D printing applied sciences are riding their use to satisfy clinical wishes, and this ebook presents an summary of those applied sciences and their integration. It exhibits how nanotechnology can raise the medical potency of prosthesis or man made tissues made by means of bioprinting or biofabrication. scholars and execs will obtain a balanced review of proper know-how with theoretical starting place, whereas nonetheless studying in regards to the most modern printing techniques.
- Includes medical purposes, regulatory hurdles, and risk-benefit research of every technology.
- This ebook will help you in selecting the right fabrics and making a choice on the suitable parameters for printing, plus contain cells and biologically lively brokers right into a published constitution
- Learn some great benefits of integrating 3D printing and nanotechnology that allows you to enhance the protection of your nano-scale fabrics for biomedical applications
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Extra resources for 3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine
2 provides a summary of the biomanufacturing techniques listed with corresponding biomaterials used. 6 A schematic representation of SLA: (a) top–down approach, (b) bottom–up approach. 1 POWDER-TYPE MATERIALS Selective laser sintering has been used with powdered materials—mostly synthetic polymers, polymer composites, or ceramics—to fabricate 3D solid structures. , 2009). Polyetheretherketone (PEEK) is a bio-inert polymer with a melting point of 343 °C and a glass temperature of 143 °C. These properties make the polymer stable to be processed at high temperatures; therefore, PEEK is easily sterilized in autoclave or by radiation without sacrificing its materials.
2010). Gold was sputter-coated on the donor slide in 55–60 mm thickness, and then the cell suspension was covered in ∼65 mm thickness on gold layer. 15 J/cm2 laser fluence. There was no significant difference in viability between laser-printed cells and control (cells without laser exposure). No damage to DNA was observed. ALP activity was measured to evaluate and compare the osteogenic differentiation between laser-printed cells and control. Immunofluorescence staining and Alcian blue staining were conducted to detect the presence of type II collagen and aggrecan, and to quantify sulfated glycosaminoglycan (sGAG).
Human osteosarcoma cells were patterned using a similar printing mechanism by Barron et al. , 2005). Immunocytochemical staining was utilized to investigate heat shock protein (HSP) expression. The expression of mouse IgG anti-HSP60 and anti-HSP 70 represented no significant cell impairment. , 2008). 35% methylcellulose) was applied on the metal or metal oxide layer. Nd:YAG laser was used with a setup of 266 nm wavelength and 4 mJ laser energy. A glass substrate was coated with Matrigel® and translated at the maximum speed of 75 mm/s.
3D Bioprinting and Nanotechnology in Tissue Engineering and Regenerative Medicine by Lijie Grace Zhang, John P Fisher, Kam Leong