By W. W. Christie
This can be the 3rd quantity of an occasional sequence of overview volumes facing points of lipid technique. As with the 1st volumes, subject matters were chosen which were constructing speedily lately and feature a few significance to lipid research. The authors are all major foreign experts.
Topics lined contain: research of positional isomers of glycerolipids through non-enzymatic equipment, separation of phospholipid sessions through high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid section behaviour and lipid diffusion, between others
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Extra info for Advances in Lipid Methodology. Volume 3
Buchnea,D. ,J. Biol. , 250, 3414-3418 (1975). 68. , In Handbook of Lipid Research, Vol. 1, Fatty Acids and Gklycerides, pp. 123-196 (1978) (edited by A. Kuksis, Plenum Press, Inc, New York). 69. J. , Can. J. , 57, 117-124 (1979). 70. , Marai,L. , Anal. , 50, 557-561 (1978). 71. , Yang,L-Y. , Biochem. , 65, 811-821 ( 1987). 72. N. , Lipids, 25, 33-39 (1990). 73. , Lipids, 19, 56-59 (1984). 74. , Lipids, 20, 778-782 (1985). 75. , Lipids, 23, 140-143 (1988). 76. , in Advances in Lipid Methodology - One, pp.
84 •Phosphatidylcholine (PC) is the internal chemical-shift reference; benzene-d6 provided the reference for field-frequency stabilization. Chemical-shift values are reported relative to 85 % orthophosphoric acid. bAtpH6. cTentative assignment. 0 . 5, corresponding to the ionization of the ethanolamine and serine residue amino groups. Sample pH can be used to distinguish between phospholipid monoesters and diesters. For example, to distinguish between lysophosphatidic acid (LPA) and lysophosphatidylglycerol (LPG), raise the pH of the solvent to 8.
Therefore, Takagi and Ando  recommend that the amount of each acyl group in the sn-1- and sn3-positions is calculated from the concentration of each component in the sn-1(3)positions and GLC analyses of sn-1- and sn-3-monoacylglycerols resolved by HPLC on the chiral column: %[sn-l] = 2 x [sn-1(3)-] x ([sn-l]/[sn-1] + [sn-3]) %[sn-3] = 2 x [sn-1(3)-] x ([sn-3]/[sn-l] + [sn-3]). g. for each component: % [sn-1(3)] = 3 x TG x [l-MG]/(2 x [1-MG] + [2-MG]) where TG, 1-MG and 2-MG represent the proportions of each acyl group in the original triacylglycerols, 1-monoacylglycerols and 2- monoacylglycerols, respectively.
Advances in Lipid Methodology. Volume 3 by W. W. Christie