By Harold H Trimm
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Extra info for Analytical chemistry : methods and applications
Based upon the performance of the mass spectrometer, the error between the observed and calculated masses was limited to 5 ppm or less. MS/MS analysis indicated the presence of two trifluoromethyl groups, so the number of atoms of F required was set to six. MS/MS analysis also indicated the presence of two acetyl groups, so the minimum number of atoms of O required was set to two. Consideration of the synthetic pathway for AMG 517 suggested that a molecule containing less than four atoms of N was unlikely.
Moreover, the accuracy of the proposed spectrofluorometric method was evaluated by the recovery studies of spiked human plasma samples. 2% (Table 4). These recovery results of the spiked human plasma indicate the suitability of the proposed spectrofluorometric method for the analysis of AMD in human plasma. Table 3. The precision of the proposed methods at three concentration levels of AMD. Table 4. Recovery studies for the proposed spectrofluorometric method for the determination of AMD in spiked human plasma.
Thousand Oaks, Calif, USA). HPLC Analytical-scale chromatographic analyses were performed on an Agilent (Wilmington, Del, USA) 1100 series HPLC system. 1% TFA in ACN. 6 mm, at 30°C) was used for the separation and quantitation of the AMG 517 impurities. Two different gradients with different flow rates were employed for the separation of AMG 517 and N-acetyl benzothiazole (see Table 1). A UV detection wavelength of 254 nm and an injection volume of 30 µL were used in the analysis of both compounds.
Analytical chemistry : methods and applications by Harold H Trimm